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Objective:To investigate the effects of Ligustrazine (TMP) on the expression of caspase-3 and ABR threshold in guinea pig cochlea after gentamicin (GM) ototoxicity. Methods:A total of 80 guinea pigs were randomly divided into saline group, GM group, GM+TMP group and TMP group. The duration of treatment was lasted 10 d continuously in all groups. The dose was decided by their weight. The ABR threshold were measured before and after administration of 10 d. SABC immunohistochemical method was used to detect the expression of caspase-3 in the spiral ganglion,cochlea hair cells,and stria vascularis of guinea pigs. Results:(1) The average gray value in the spiral ganglion cells, stria vascularis and hair cells in GM group had significant difference with those in the same position of control group (P<0.05) .The average gray value in the spiral ganglion cells and hair cells in GM+TMP group had no difference with those in the same position of control group (P>0.05), but in stria vascularis (P<0.05) . Meanwhile the average gray value in spiral ganglion, hair cells, stria vascularis in TMP+GM group had significant difference with those in GM group (P<0.05) . (2) The ABR threshold had no significant differences before treatment (P>0.05) . After administration of 10 d, the ABR threshold of GM group was significantly changed (P<0.05) . The ABR thresholds in GM group was significantly higher than that in control group (P<0.05) . The ABR threshold in TMP+GM group was lower than that in GM group (P<0.05) . Conclusion:TMP participates in preventing the process of cochlea injury caused by GM,through blocking the expression of caspase-3 in cochlea tissue of GM ototoxicosis probably,which achieve the protection of GM deafness eventually.
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0.05). 100% occlusion was achieved in 18 patients with cerebral aneurysms by using embolization. Conclusion 3D DSA may improve the accuracy in diagnosing SAH and in showing clearly the stereo conformation of aneurysm and the relationship of sac and parent artery. It is helpful in the evaluation and guidance of embolization of cerebral aneurysms.
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Objective To investigate the curative effect and complications of intra-arterial thrombolysis (IAT) with recombinant tissue plasminogen activator(rt-PA) for patients with acute ischemic stroke, and analyse the factors related the outcomes.Methods 12 patients were treated by IAT with rt-PA in our hospital from Oct.2002 to Oct.2003. Primary neuroradiological assessment was performed with CT in all patients. Mechanical disruption of clot remnants were attempted after rt-PA infusion. Angiographic recanalization was classified according to thrombolysis in myocardial infarction (TIMI) grades. Clinical evaluation was undertaken 20 d after thrombolysis with classification of modified rankin scale (MRS) scores, good for 0 to 3 and poor for 4 to 6. Results Before thrombolysis the scores for TIMI 1 in 1 case was 8.33% and TIMI 0 in 11 cases was 91.67%. The rates of complete or partial recanalization just after IAT were 75%(9/12), less or no recanalization were 25%(3/12). Good outcome in 66.7%(8/12), poor outcome in 33.33%(4/12). Cerebral hemorrhage occurred in 1 case.Conclusions Intra-arterial thrombolysis ( IAT) with rt-PA is feasible and safe in treatment of acute ischemic stroke.